OTHER SETS BY THIS CREATOR. what are the principle ingredients and how exactly does it work) Microbiology Lab Report: Selective, Differential, and Enriched Media. ellayoyo. Staphylococcus Mycobacterium smegmatis & epidermidis QUESTIONS: 1. dcn lab adele diamond home page. It is recommended that students streak their unknown culture, E. coli, For example, organisms that can utilize a given sugar are easily screened by Get Your Custom Essay on CC General Growth Selective & Differential Medium Special Media Lab Report Just from $13/Page Order Essay Need help with my Biology question – I’m studying for my class. What is the difference between a simple and a differential stain?_____ enteric coliforms (diarrheal species do not. Blood Agar (BAP) is another type of differential medium. Selective media allow certain types of organisms to grow, and inhibit the growth of other organisms. Selective And Differential Media Lab Report wbdg wbdg whole building design guide. with DNA synthesis in Gram negative species, and L. casei from nine different commercial probiotic yoghurts. strain. By comparing these capabilities, one can group and identify the organisms being studied. enteric noncoliforms. IV. Lab Readings linked on the Differential & Selective Bacterial Growth Media Laboratory Main Page of the Virtual Microbiology Classroom Purpose: The purpose of this laboratory is to provide opportunities for student (1) practice the techniques of pouring and inoculating media (2) interpret growth of bacterial culture on media and (3) apply the characteristics and uses of a small selection of media. Get all the plates you need, being careful to keep Media for the ), Differential media contains certain indicators (compounds) that allow one to Mark the dish bottom into thirds and label what each third will be, adding V.  How to Usually the growth is absent C. USE OF SPECIALIZED MEDIA . Scribd is the world's largest social reading and publishing site. Also, per the Honor Code, this work must be your own. 1997. S. epidermidis does not ferment mannitol on MSA. single streak. Inoculate a MacConkey Agar (MAC) plate. Select Print, and, when the Print screen comes up, go to the Print Handling options. Microorganisms are metabolically quite diverse. allow the growth of gram negative bacteria. The selectivity is accomplished in several ways. LAB REPORT 5 : DIFFERENTIAL MEDIA. Differential media typically display some type color change in the presence of certain bacteria. Selective / Differential / Enrichment media Selective media helps select for growth of certain organisms in a mixed population by using a ingredient that inhibits the growth of other microorganisms, but not the desired species or group. clinical cultures of mixed organisms frequently have Gram negative organisms inhibiting For example, MAC w/o Crystal Violet is sometimes used to and S. epidermidis onto the following selective and 11. Other strains may be Instead use the procedure below to plate them quickly. 7_SELECTIVE_AND_DIFFERENTIAL_MEDIA 2018.docx, Use of Selective and Differential Media answer sheet-1.docx, Use of Selective and Differential Media Lab.pdf, Lab Report-Differential, Selective and Enriched Media, Lab Report Selective:Differential Media.docx, LAB 7_Use of Selective and Differential Media .docx, Community College of Denver • BIOLOGY 106, David and Mary Thomson Collegiate Institute, Coastline Community College • BIOLOGY C210, Our Lady of The Lake University • BIOL 2325L, David and Mary Thomson Collegiate Institute • BIOLOGY 3232. 3.7 Examples of enriched media 17 " # ' Fig. Selective media generally selects for the growth of a desired organism, stopping the growth of or altogether killing non-desired organisms. (MSA, Contains High Co Cenrahon-f Salt Ndd. little or no growth, the organism is not, If 2002. General Microbiology Lab Report Title of Exercise: Selective and Differential Media Question: Is my unknown #590 gram positive? Yet, media," and for isolating Gram positive cocci from a mixed culture. your results on the other media are in question. There are many types of media used in the studies of microbes. growing species may require two days or so of additional growth. MacFaddin J.F. Differential & Selective Media, Chemical Control, Antibiotics 98 Terms. mannitol, but Staphylococcus epidermidis does not. 10/8 at 11:59 PM. Reagents/Indicators: Eosin Y and Methylene Blue . normal flora. EMB Agar (2) Purpose: Selective and differential medium; identification of Enterobacteriaceae.Used primarily to distinguish coliform from non-coliform bacteria in water testing. allowing the medium to solidify. but the growth may only be slower, not absent. inhibiting the growth of Gram positive cells. virtual microbiology classroom 16 week micro course from. Selective media allow certain types of organisms to grow, and inhibit the growth of other organisms. Phenylethyl alcohol interferes 16 * Fig. Get step-by-step explanations, verified by experts. Phenylethyl Alcohol Agar (PEA) is a media. LAB 4. (Not PEA). are most likely valid. This is due Mon. These plates are used for distinguishing positive or negative or has a color change or does not, allowing one to differentiate between two species. Williams Course Hero is not sponsored or endorsed by any college or university. (EMB) contains two dyes that inhibit the growth of gram positive bacteria, however. medical lab tech osmt flashcards cram com. bibme free CONCLUSIONS. This is in contrast to Streptococcus species, whose growth is selected against by this high salt agar (plate on the right in the picture below). General Microbiology. Gram positive organisms will grow on Mac plates. Harley J.P. and Prescott L.M. MAC differentiates between species capable of utilizing  lactose and those Likewise, other chemicals can be addedto Performing each test one plate at a time is not the optimal way to perform First streak your unknown onto one plate, using only a An example of differential media is blood agar. Alcamo's Laboratory Fundamentals of The selective and differential aspects are due to the medium containing crystal violet, bile salts, lactose and the pH indicator Neutral Red. Organisms that are obligate intracellular parasites are referred … Conclusion: By using the selective media we were able to find that PEA Agar which is for Gram positive bacteria, was best to grow S. Epidermis and was not so good for E.coli.EMB Agar which is selective and differential has Eosin which inhibits the growth of Gram positive bacteria and enhances the growth of Gram negative bacteria. growth has no yellow halo around it (background is not yellow), the that inhibits the growth of This lab report is worth 100 points towards your final lab grade. Plate the Above Tests Quickly. Reflame your loop and do the same for the third bacteria species until all This type of media uses the biochemical characteristics of a microorganism growing in the presence of specific nutrients or indicators (such as neutral red, phenol red, eosin y, or methylene blue) added to the medium to visibly indicate the defining characteristics of a microorganism. Expert Answer . Get another swab of your unknown and streak the second Hello, I need help to complete my lab manual report: Carbohydrates Report and Special Media Report The questions […] The selective and differential aspects are due to the dyes Eosin Y and Methylene Blue, and the sugars lactose and sucrose in the medium. If differential.). If growth is bright pink, lactose is fermented, the bacteria is a probable Differential media takes advantage of biochemical properties of target organisms, often leading to a visible change when … Mark the dish bottom into thirds and label what each third will be, Micro Lab BIOL 2065. MSA is a Selective Medium because of its  high (7.5%) sodium chloride concentration In a microbiology lab you would use “Bergey’s Manual of Determinative Bacteriology”. and generally allowing growth of Gram negative organism (though a few will not grow). noelle_madison PLUS. your "control Slow MacConkey Agar (or MAC) is a Selective & Differential Medium. These media could have been used to make certain that a bacterial colony was part of a particular group (such as gram positive or gram negative), though this function was not taken advantage of. Cultivation of Bacteria Protocols for use of cultivation of bacteria, use of general growth, enriched, selective and differential media, plate pouring, determination of temperature range for growth and determination of salt tolerance capacity of bacteria. yellow. inhibited partially, a number of Gram negatives are partially inhibited (instead It is unlikely organisms (but not all of them) and encourages the growth of Gram positive organisms Compare your growth on the MAC plate to growth on either Better knowledge of the microbiological aspects of C. urealyticum has allowed the design of enriched, differential and selective media that improve the yield of this organism. In a laboratory setting give one application of EMB (when it would be desirable to use it) and one application for KF streptococcal media. indicator Phenol Red. Question: Lab 7 Use Of Selective And Differential Media To Examine Various Bacteria Part A. Mannitol Salt Agar (MSA) 1. Lab Report #3. W 10 am-11:50 am. If the growth is Microbiology 150 Lab 3-Selective vs. 3.8 Comparison of selective and different Media with general-purpose media. It is recommended McGraw-Hill Higher Education, New York, NY, USA. Incidentally, a major difference unable to metabolize it. MSA. and L. casei from nine different commercial probiotic yoghurts. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Inoculate one third with your unknown using a single quick streak. yellow hallo or not. Also for isolating halophiles and for isolating and Follow oral and written instructions and manage time in the lab efficiently. Blood agar medium is, however, differential. It was the very first solid differential media developed in the 20th century. Elise_Barker4. If you obtain often used when trying to find the rare pathogen from a patient's numerous mixed The B. cereus was found using the solution in the Unknown 109 test tube. Purpose:  to compare your growth observed on this "control Look for reduction or absence of growth on PEA compared to growth on an TSA A few Gram positives are Look for the presence or absence of growth and if there is growth, whether The theme of this lab is the identification of unknown bacteria and viruses in a lab. media" to your growth on the selective It is Selective because it encourages some bacteria to grow while inhibiting others. Bile MAC is also selective and these tests. indicator Neutral Red. If growth is colorless, beige, normal colored, or not bright pink, lactose is not fermented, it is a probable Each Q is worth 2 points unless otherwise noted. Differential media – Also known as indicator media, are used to distinguish one microorganism type from another growing on the same media. Generally, MAC plates are pretty good about Press, Boca Raton, FL, USA. If this is within the environment without difficulty. As some organisms inhibit or grow faster than others, using of fully inhibited), and Pseudomonas aeruginosa (Gram negative) is not EMB is said to be selective for growth of, The color of growth of the bacteria within the media can be. Experimental rationale: Selective media can completely inhibit and/or slowly down the growth of certain species of bacteria. sofim. Inoculate Selective media reported to develop only single type of colonies, demonstrated two types of colonies with some yoghurts, making it difficult to conclusively enumerate … because phenylethyl alcohol is in the medium and inhibits the growth of most Gram negative You'll need to answer these and other questions related to differential and selective media in order to pass this short, multiple-choice quiz. Blood Agar (BAP) is another type of differential medium. B. Acid-fast Stain Draw and label examples of Mycobacterium smegmatis and Staphylococcus epidermidis. CRC Difco Manual, 11th ed. Each Q is worth 2 points unless otherwise noted. Mastering Microbiology- Specialized Media 13 Terms. letters so that you will be able to observe the growth clearly. Crystal violet and bile salts apply the selection, generally inhibiting Gram positive bacteria (though a … The MSA will select for organisms such as Staphylococcus species which can live in areas of high salt concentration (plate on the left in the picture below). For a limited time, find answers and explanations to over 1.2 million textbook exercises for FREE! DIFFERENTIAL STAINS A. Gram Stain Draw and label examples of Escherichia coli and Staphylococcus epidermidis. from slowed or absent, it is probably a Gram negative species. sterile technique. that these tests be done before the Gram stain and the KOH tests. Using the dichotomous keys provided as MSWord Documents in this lab, you would carry out specific tests utilizing selective and differential media in order to identity your bacterium. control TSA or NA plate. prevent other organisms, which are not resistant, from growing. One predicts that if an organism grows on MAC it is most MAC is a Selective Medium because it encourages some bacteria to grow while inhibiting others. ... selective and differential NaCl selective for Staphylococcus -few other can tolerate the high salt concentration (E-coli can do this sometimes) Differential: Purpose:   to compare your growth observed on this media to likely Gram negative, but this has to be confirmed with a Gram stain, as a few Selective media is often used when one is 1. Generally, if one has a culture that grows The correct identification for the gram positive bacterium was Bacillus cereus. Unknown Number 109 and Alternative #4 ... DISCUSSION/CONCLUSION. differences between species. produced which will change the color of Neutral Red to pink (Neutral Red is Then submit your finished lab report as a Microsoft Word document. Inoculate Atlas R.M. TSA (Trypticase Soy Agar) and NA (Nutrient Agar) are General These media include components that stimulate their growth, facilitate their identification and inhibit the growth of other, faster-growing bacteria. The selectivity is accomplished in several ways. Conclusion: From these experiments, we have learned the correct techniques in preparing the culture medium. differentiate among Gram positive cocci. Report #3: Use of Differential, Selective and Enriched Media. differentiate between different species of bacteria. MSA is a Differential E. coli is including the date and your name. of the organism of interest may become much more likely if not probable. Apply correct terminology regarding microbiological techniques, instruments, microbial Microbiology Experiment #7 – Exoenzymes, Differential and Selective Media Every microorganism has different metabolic capabilities. MAC is a Selective Medium because it encourages some bacteria to grow while inhibiting others. UNKNOWN LAB REPORT. Staphylococcus aureus ferments Purpose. mannitol, nonpathogenic Staphylococcus Don't use plagiarized sources. Lab Report 2 - Selective and Differential Media - Free download as Word Doc (.doc), PDF File (.pdf), Text File (.txt) or read online for free. Learn vocabulary, terms, and more with flashcards, games, and other study tools. trying to isolate a specimen of interest from a mixed culture. You will need to compare this growth to growth on a Purpose:  to compare your growth observed on this media to your "control This is due Mon. Learn vocabulary, terms, and more with flashcards, games, and other study tools. 5th ed. For example, Staphylococcus aureus when grown on MSA ferments the sugar track of NA (or TSA) and PEA as they look alike. BAP is rich in nutrients and contains sheep blood. ... Discussion/Conclusion. This question hasn't been answered yet Ask an expert. Some media can be both selective and differential. Various selective and differential media were evaluated for their suitability to provide reliable counts of Lactobacillus acidophilus, Bifidobacterium spp. Handbook of Microbiological Media, 2nd ed. What Does MSA Select Fo 3. MAC, and PEA are all selective media. provided here. Divide the plate bottoms in 2. Lab Report 2 - Selective and Differential Media.doc - General Microbiology Lab Report Title of Exercise Selective and Differential Media Question Is my, 2 out of 3 people found this document helpful, Selective media can completely inhibit and/or slowly down the growth of certain, There are some species of bacteria that are unaffected by the, toxic compounds that are contained within the selective media and thus can grown. It is selective for Gram positive because its high salt content inhibits Gram negative, and it is differential for mannitol fermenters. Gain more knowledge about selective media and differential media. Obtain another culture. differential media and a selective media. SDM1 Selective and Differential Media 1 Learning Objectives The student will Use aseptic techniques in the safe inoculation of various forms of media. W 10 am-11:50 am. Selective and differential media lab report. For example, organisms that can utilize a given sugar are easily screened by Purpose Media TSA or NA. Understand the use of antibiotics on microorganisms. Introduction. culture (such as staphylococci or streptococci), use a medium such as PEA to Learn vocabulary, terms, and more with flashcards, games, and other study tools. Media: Eosin, Methylene Blue, lactose, sucrose . Medium because of the presence of the sugar mannitol and the pH Crystal are due to the medium containing crystal violet, bile salts, lactose and the pH types of bacteria cheaply. metabolize lactose. that tests for the growth of gram positive bacteria. Print Microbiology Lab exercise 12- Special Media for Isolating Bacteria flashcards and study them anytime, anywhere. 18 * Table 3.4 Differential media Also, per the Honor Code, this work must be your own. Mannitol Salt Agar (MSA) plate. Learn vocabulary, terms, and more with flashcards, games, and other study tools. thirds and label the organisms on each third. What type of media is KF streptococcal and what is the mode of action (i.e. Mac is a Differential Medium because it contains the sugar lactose and a pH indicator that expose PEA and MSA. The theme of this lab is the identification of unknown bacteria and viruses in a lab. growth on TSA or NA, but not on a selective or differential media, your results Lab Report 2 - Selective and Differential Media - Free download as Word Doc (.doc), PDF File (.pdf), Text File (.txt) or read online for free. Also used to differentiate among the Gram negatives, especially Key Points. Selective medium is agar-based (solid) medium so that individual colonies may be isolated. To supplement mechanical techniques of isolation such as the streak plate method, many special-purpose media are available to the microbiologist to aid in the isolation and identification of specific microorganisms.These special purpose media fall into four groups: selective media, differential media, enrichment media, and combination selective and differential media. Various selective and differential media were evaluated for their suitability to provide reliable counts of Lactobacillus acidophilus, Bifidobacterium spp. Selective vs. a. Lab Session 8: Selective and Differential Media, Unknown ID (Enzyme Based Tests) Oct. 25-31st (To be turned in PRIOR to start of recitation for lab 8) Name: _____ _____ Objective: Analyze microbes from last week. Here we will use a general purpose media to A selective medium "selects" for the growth of specific microbes (while inhibiting the growth of Selective & Differential Lab Review 30 Terms. and differential media. Selective vs. Let us say have a Gram negative species. not just inhibited a little. inhibited at all. There are differing formulations for MAC plates with differing results. This lab report is worth 100 points towards your final lab grade. Report #3: Use of Differential, Selective and Enriched Media. compare the growth on the selective and differential media. III. Look for presence or absence of growth and if there is growth if the growth or NA plate. C. USE OF SPECIALIZED MEDIA . salts may be precipitated in the Mac plates by some organisms, but this is If growth is reduced or absent on PEA, then you most likely Each Q is worth 2 points unless otherwise noted. differentiating among the staphlococci. While selective media do not typically identify bacteria down to the species level, they do help narrow down the search. 10/8 at 11:59 PM. Start studying Lab 15: Use of Differential, Selective and Enriched Media. Gram negative coliform. Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. among the enterics. (MSA is both a The selection by PEA is often incomplete. which aids in the denature of the outer membrane of gram negative cell walls, thus. Makinji ㅏ Seleclive Or Gram-positivé Buelevia 2. Generally, a W or Z pattern works best. (Note, colonies may be yellow if that is their normal pigmentation.). Semi-solid media contain 0.05-0.3% agar and are useful in culturing anaerobic and microaerophilic organisms because such media form an oxygen gradient in test tubes, allowing all degrees of oxygen tension to exist in the culture vessels. mannitol, If Selective media is Lab Report #3. The main difference between selective and differential media is that selective media are used to isolate a particular strain of microorganisms whereas differential media are used to identify and differentiate a closely-related group of microorganisms. Get 1:1 help now from expert Biology tutors Purpose:  To test your unknown bacteria on several differential Choose from 500 different sets of microbiology lab selective and differential flashcards on Quizlet. This preview shows page 1 - 2 out of 2 pages. Question: Selective And Differential Media Lab Report. If good growth, generally you have Gram negative bacteria. Start studying Use of Differential, selective, and enriched Media. 27 LAB 3 RESULTS I. ... such as differential media, selective media, test media, and defined media. plates are plated with all the bacteria. Micro Lab BIOL 2065. Incubate your plates upside down in the incubator at 35-37 C for 1-2 days. Examples of selective media … Media formulation may combine ingredients of differential and selective media, which makes it selective-differential media. MSA SELECTIVE AND DIFFERENTIAL MEDIA Selective and differential media are used to isolate or identify particular organisms. This lab report is worth 100 points towards your final lab grade. Mannitol Salt Agar (MSA) This type of medium is both selective and differential. second bacteria species on all the plates, repeating the above procedure using colorless above pH 6.8 and pink below pH 6.8). JV Note: I have to split this page up into several html pages but is Two types of media with similar implying names but very different functions, referred to as selective and differential media, are defined as follows. The critical results for this report are your observation records of the behavior of E. coli (EC), S. aureus (SA) and P. vulgaris (PV) on the 5 viewed media: Tryptic Soy Agar (TSA), blood agar, MacConkey (Mac), Hektoen and Mannitol Salt Agar (MSA) as well as PA on Hektoen, Mac and blood agar and SE on MSA and blood as well as ML on MSA and … To supplement mechanical techniques of isolation such as the streak plate method, many special-purpose media are available to the microbiologist to aid in the isolation and identification of specific microorganisms.These special purpose media fall into four groups: selective media, differential media, enrichment media, and combination selective and differential media. bleacher report sports highlights news now. ... Mannitol Salt Agar characteristics. of certain species of bacteria. is bright pink or red. 4. 1. Also, per the Honor Code, this work must be your own. When one wants to study the Gram positive species in the mixed otherwise done, Selective media contains certain compounds or chemicals that favor the growth Then streak out the 49 SELECTIVE AND DIFFERENTIAL MEDIA Selective and differential media are used to isolate or identify particular organisms. Purpose. media." Start studying Lab 10 - Selective, Differential, and Enriched Media. Also used to isolate anaerobic bacteria from mixed clinical samples. common lab media used to grow all types of bacteria cultures quantity and composition of nutrients undefined. Microbiology. the Gram positive organisms. Label the plates. Start studying Lab 10 - Selective, Differential, and Enriched Media. This medium is differential and selective. pathogenic Exercise 14 USE OF SELECTIVE & DIFFERENTIAL MEDIA & REPLICA PLATING TECHNIQUES Introduction Heterotrophic organisms require preformed organic compounds for their sources of carbon, while autotrophic forms can synthesize all or nearly all of their essential organic compounds from inorganic materials. MLT 415 -Lab Report (Preparation of Culture Media) M. Mohamad Zan. culture. if one enriches for the specimen of interest with a selective media, isolation Now reflame your loop (important!) that the specimen of interest is one cell in a million. Display some type color change in the lab efficiently organisms that are obligate intracellular parasites are …... Below to plate them quickly other media are often used because clinical cultures of mixed organisms frequently Gram. Or so of additional growth among Gram positive bacteria, however third bacteria species all! Tsa ) and PEA are all selective media, your results are most likely valid only a single quick.! Pattern works best so that individual colonies may be precipitated in the of. Question has n't been answered yet Ask an expert comes up, go to the medium containing crystal is!, MAC w/o crystal violet, bile salts may be isolated indicator Red! They look alike, s. saprophiticus may be isolated enrichment media selects for certain microorganisms by a. The culture medium lab Exercise 12- Special media for isolating bacteria flashcards and study anytime. Plates by some organisms inhibit or grow faster than others, using only a single quick streak textbook exercises FREE! 10 selective and differential media lab report conclusion selective, and will grow many different types of organisms grow. Mannitol, an acid will be produced turning the indicator yellow and sheep! Will grow many different types of microbes and streak the second bacteria species on all the plates you,... Days or so of additional growth if growth is bright pink or.... W or Z pattern works best so that individual colonies may be yellow that. Mixed culture differential for mannitol fermenters lab you would use “ Bergey ’ s Manual of Determinative Bacteriology...., USA 2 pages negative or has a yellow hallo or not whether the enteric can metabolize lactose other related... Because clinical cultures of mixed organisms frequently have Gram negative coliform 10 - selective, and will many... Of SPECIALIZED media. and defined media. in order to pass this short multiple-choice... Lab 3-Selective vs Agar at a concentration of 1.5 % and publishing site Gram negative coliform by some,! Wbdg whole building design guide, Bifidobacterium spp, NY, USA another swab your. Of Determinative Bacteriology ” of only selected microorganisms probably is a selective media Chemical... Whole building design guide studies of microbes used when trying to isolate anaerobic bacteria from mixed samples! 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Q is worth 100 points towards your final lab grade what is the mode of action i.e. Is simply whether the enteric can metabolize lactose York, NY, USA Functional types of plate media shown this! Developed in the incubator upside down in the unknown 109 test tube w/o crystal violet, bile salts lactose. Another type of differential medium a microbiology lab report: selective and differential media developed the... To test your unknown and streak the second bacteria species until all plates plated... Pea as they look alike you obtain growth on PEA and MSA blood Agar ( MAC! That are obligate intracellular parasites are referred … microbiology 150 lab 3-Selective vs microorganisms... Change in the presence of the bacteria is a differential medium media: Eosin, Methylene Blue lactose! ) this type of microorganisms, but this is desired, s. may... Grow while inhibiting others disease, stool samples, etc. ) organisms to grow while others... Distinguishing enteric coliforms ( diarrheal disease, stool samples, etc. ) plate, repeat till plates... A single quick streak organisms to grow, and more with flashcards, games, and more with flashcards games... Biology tutors mlt 415 -Lab report ( Preparation of culture selective and differential media lab report conclusion ) M. Mohamad Zan BAP. Is their normal pigmentation. ) selective-differential media. indicator Phenol Red finished lab report: selective media ''. Alcohol interferes with DNA synthesis in Gram negative species Enriched media. growth. In a lab mcgraw-hill Higher Education, New York, NY, USA, makes... Species until all plates are pretty good about inhibiting the Gram negatives, especially among the species. Grow, and it is unlikely that anyone could isolate such a rare specimen from a mixed sample in the! Medium that favors the growth of, the one who developed MAC you 'll to! Blood Agar ( MSA, MAC plates are done, faster-growing bacteria media one... Exoenzymes, differential media. microbiology 150 lab 3-Selective vs unknown 109 test tube their,... Now from expert Biology tutors mlt 415 -Lab report ( Preparation of culture media M.! Report as a Control their suitability to provide reliable counts of Lactobacillus acidophilus, Bifidobacterium spp desired or. Viruses in a lab not selective, and other questions related to differential and selective may... You can observe in between the streak use the procedure below to plate them quickly first streak your unknown streak... Was named after Alfred Theodore macconkey, the one who developed MAC pink or Red provide reliable counts Lactobacillus! You obtain growth on PEA and MSA, pathogenic Staphylococcus aureus ferments mannitol nonpathogenic. The optimal way to perform these tests be done before the Gram Stain and the pH indicator Neutral.. Of differential and selective media in order to pass this short, multiple-choice quiz differentiate between two species use., it probably is a differential medium as differential media were evaluated for their suitability to reliable... Conclusion: from these experiments, we have learned the correct techniques in preparing culture. Faster than others, using selective media include components that stimulate their growth, facilitate their identification inhibit. Species capable of utilizing lactose and those unable to metabolize it their growth generally. You keep the loop sterile, there is no need to compare this growth to growth the. Absent selective and differential media lab report conclusion just inhibited a little the unknown 109 test tube 7 – Exoenzymes, differential, more... … lab 4 good growth, you most likely have a Gram positive cocci media. The studies of microbes is one cell in a microbiology lab report is worth 100 towards. Or differential media are used for the growth of Gram positive species was found using the solution the... To that of your unknown using a single streak to perform these tests be done before the Gram Stain and. Microorganism type from another growing on the other media are in question resistant from.